Hit to Leads

Services for Hits-to-Leads discovery

• In silico models for ADME properties
• In silico models for biological activity and toxicity predictions
• Experimental physico-chemical property analysis
• Plasma protein binding
• In vitro permeability in PAMPA, Caco-2 and MDCK
• CYP identification, inhibition and induction
• Metabolite identification and stability

Other expertise areas

• SBW Solutions
• SBW-Alpha - Target Discovery
• SBW-Beta - Hit screening
• SBW-Gamma - Hits to leads
• SBW-Delta - Lead optimization
• SBW-Epsilon - Pre clinical
• SBW- Zeta - Clinical development
• Food industry
• Clinical development phases 3-4

• Re-testing of the hits in the same conditions as in the HTS program as well as confirmation by an alternative testing (orthogonal testing)
• Secondary screening by cellular assays
• Generation of IC50 and EC50 values
• Synthesis feasibility and cost analysis
• Patentability checking
• Assessment of the biophysical binding mode of the ligand to the target, using technologies NMR and surface plasmon resonance
• Development of the Structure-Activity-Relation models

As the confirmed hits are developed further, further properties are characterized, initially like

• Chemical stability
• Physicochemical properties
• Possible binding to plasma proteins
• Possible inhibition or induction of CYP P-450 enzymes
• Cell membrane permeability
• Possible cytotoxicity
• Kinetics of the metabolism
• Target selectivity

SBW Platform for Hits-to-Leads discovery

• In silico models for ADME properties
• In silico models for biological activity and toxicity predictions
• Experimental physico-chemical property analysis
• Plasma protein binding
• In vitro permeability in PAMPA, Caco-2 and MDCK
• CYP identification, inhibition and induction
• Metabolite identification and stability

All our services are tailored per customers need. Our business models includes variations from fee for service / FTE to strategic business process outsourcing and partnership. Please see this page for our business models and here for an overview of our various solutions areas.