In vitro genotoxicity

Related services

• Toxicity and safety models
• Metabolites in safety testing (MIST)
• CYP identification, inhibition and induction
• Metabolite identification and stability
• Pre-clinical pharmacokinetics
• ONTOMINE - In silico bioactivity, adverse effect and toxicity screening for small molecules and metabolites
• ONTOMINE ADME - In silico models for logP, logD, logS, pKa

Ames Test (Bacterial Reverse Mutation Test)

The Ames test is used to evaluate the mutagenic properties of test items. The test uses histidine dependent bacterial strains (S. typhimurium and/or E.coli). In the absence of an external histidine source, the bacteria cannot grow. Bacterial growth is resumed by a reversion of the mutation, allowing the production of histidine to be resumed. The genotoxicity is assessed as the ability to increase the number of revertant colonies relative to background (spontaneous revertants) level.

The Ames test is the most widely used regulatory test for genotoxicity and is required for IND submission. A positive result form the Ames provides almost certain result of a compound being genotoxic. However, a negative Ames result does not assure the compound not being genotoxic due to e.g. lacking elements of genotoxicty target in the prokaryotic cells.

We offer the AMES test as three variants, with different number of S. typhimurium strains, replicates and controls used

• Screening-AMES test is most suited for early genotox screening of large number of compounds
• Ranking-AMES is to rank smaller number of compounds in later phases of drug discovery
• Full-AMES is most comprehensive AMES test, conducted according to the OECD Guideline 471

Please follow this link to retrieve a detailed fact sheet of the AMES genotoxicity testing service

Other in vitro genotoxicity tests

In addition to Ames test, we offer the following other in vitro genotoxicity tests

• Micronucleus test
• Mouse lymphoma test

Contact us from this link.